Envoyé par : Étudiant(e) Bioinformatique
Répondu par : Béatrice Roure
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2008-01-23 |
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Hello,
I still have a lot of confusions on how to analyse the results. In fact I have used the profile sequencing(as seen in the TP) and added the new_sequence(containing sequence1,2,3 and 4) to each aligned subtype profile one at the time, resulting in 3 new fasta file. I have also calculated the score for each fasta file and displayed the low scoring one( as seen in the tp) However I am still unable to determine a precise method, in order to find the sequence with the higher homology to the other subtypes using clustalx. In addition I find that this visualization is very bias and error-prone. The question that I am trying to ask is that are there any other tools able to give me an overall , global score for the entire alignment or a more accurate way to visualize the homology in order to facilitate the task of determining the homologous or closely-related sequence. I hope my question is clear! |
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L'idée générale est correcte.
Si l'ajout des 4 nouvelles séquences en même temps ne donne pas un résultat permettant de déterminer à quel type une séquence peut être affiliée, il faut creuser l'idée afin de d'obtenir un score d'alignement différent pour chaque nouvelle séquence. Je ne peux pas en dire plus sans décrire précisément le protocole expérimental à appliquer. Béatrice |
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